Name: kawthar Abdelaziz Elsayed Mohamed Diab

 Profession:Researcher

 Affiliation:Genetics and Cytology Department,

 Genetic Engineering and Biotechnology Division,

 National Research Center.

 Work address: El-Buhouth St.. Dokki, Cairo, Egypt

Education and Research Experience :

▂  Post-doctoral fellowship, Tokai University, Department of Bioscience, School of Agriculture, Kawayo,  Aso, Kumamoto, Japan. From 15 June 2014 To 12 December 2014.

▂  Post-doctoral fellowship, Cancer Pharmacology Division, Indian institute of integrative medicine, Jammu, India, From 10 March 2012 To 8 September 2012

▂  Ph.D. Degree in Zoology, Faculty of Science, Ain Shams University, March 2009.

▂  M.Sc. Degree in Experimental Zoology, Faculty of Science, Zagazig University, May 2003

▂ B.Sc. Degree in Zoology, Faculty of Science, Al-Azhar University, May 1996.  Grade: very good

Professional Experience:

I-Genotoxicity assay ( in vitro and in vivo)

• Chromosomal aberrations assay
•  Sister chromatid exchange
•   Micronucleus assay
• Sperm analysis
• Dominant lethal mutation assay
•  Comet assay
• Analysis of DNA fragmentation by agarose gel
• Quantitation of DNA fragmentation with diphenylamine
• Morphological study for apoptosis by Fluorescent microscope

II-In vitro anticancer cytotoxicity

•  Sulforhodamine B (SRB) colorimetric assay
• MTT (Tetrazolium) colorimetric assay
• WST (CCK-8) colorimetric assay
•  NA measurement and cell cycle analysis by flow cytometry
•  DNA fragmentation assay