JOHN LOWE, PhD.

11 East Bellefonte Avenue #202  Alexandria, Virginia 22301 615-618-8636   lowe.john1@gmail.com

BIOMEDICAL SCIENTIST

Dynamic and highly motivated information scientist and biomedical research expert with over 12 years of experience directing laboratory and medical-scientific processes. Expertise in cell biology, coagulation, pharmacogenomics, molecular pathology, and cardiovascular disease. Well-versed in mammalian colony management for experimental research and data collection. Extensive background designing and implementing policy & procedure to ensure superior quality assurance, documentation, and compliance with all government / institutional protocols and regulations. Skilled communicator, able to prepare and present engaging medical / scientific discussions, meetings, and training sessions both internally and externally. Experienced change manager, identifying areas of improvement, creating effective plans, training colleagues / staff in new processes, and following through post-implementation to assess application outcomes and make adjustments for successful execution. Team player, collaborating with colleagues to create solutions, ensure understanding, and develop improved methods. Dynamic leader with proactive approach to achieving objectives, promoting cooperation and alignment across departments.

Analytical Thinking  Troubleshooting  Quality Assurance  Risk Assessment  Biomedical Expertise

Collaborative Leadership  Change Management  Integrity  Strategic Development  Communication

Operational Enhancements  Workflow Optimization  Government Compliance  Proactive Approach

Goal-Motivated  Critical Thinking  Team Builder  Adaptability  Solution Provider  Training & Mentoring

PROFESSIONAL EXPERIENCE

VANDERBILT UNIVERSITY MEDICAL CENTER, Nashville, Tennessee 2001-2013

Healthcare & biomedical education provider staffing 22K+.

Information Scientist 2012-2013

Headed expert research and resource support in didactic, pharmacogenomics, and clinical coagulation laboratory programs. Collaborated in preparing Clinical Informatics Consult Service information packets for trauma-intensive care unit. Utilized biomedical literature search strategies and content-specific knowledge to create evidence-based summary packets for Coagulation Diagnostic Management Team.

• Cut hospital costs in testing and enhanced hospital reputation by developing information packets used by Coagulation Diagnostic Management Team in clinically-challenging laboratory case decision making.

• Maintained up-to-date expert knowledge in coagulation pathways and testing technology through extensive research, proactively reviewing biomedical literature, and identifying resources to keep abreast of current trends.

Post-Doctoral Research Fellow 2008-2012

Directed 3 research assistant activities in animal husbandry (genotype, colony management), protein biochemistry / cellular imaging, and drug-challenge / electrophysiology / molecule rescue experiments. Oversaw 3 projects researching cell-surface targeting and electrophysiological attributes of cardiac sodium channel mutations discovered in patients with cardiac arrhythmia, using mouse animal model. Conducted research assistant training on laboratory techniques & experimental modalities to ensure consistent, reproducible data. Prepared and presented data findings in laboratory meetings, departmental seminars, international conferences, and in peer-reviewed academic journals.

• Enhanced data lab quantity and quality by improving protein biochemistry methods, cellular imaging techniques, and creating new strategies in policy & procedure optimization for protein biochemistry, cardiomyocyte isolation, and confocal imaging.

• Significantly boosted colleague technical knowledge by providing common vocabulary when discussing experimental findings and future experimentation, breaking down complex concepts and enabling understanding.

• Improved workflow and ensured regulatory / experimental design tactics by participating in Clinical Pharmacology weekly seminars and disseminating information at laboratory meetings.

Pre-Doctoral Research Fellow 2004-2008

Led research in cell biology using meticulous data collection and management methods. Conducted studies while adhering to institutional, state, and federal regulations. Maintained documentation in relation to laboratory protocols, ensuring compliance with IACUC, FDA, NIH, USDA, and OSHA regulations. Successfully managed complex research and performed didactic coursework in pathology, including cellular / molecular pathology, with specified focus on cardiovascular disease and physiopathology.

• Successfully completed didactic training and laboratory hours that yielded publications in peer-reviewed academic journals, and invited oral presentation at American Heart Association Scientific Sessions meeting in 2007.

JOHN LOWE  Page 2 lowe.john1@gmail.com

VANDERBILT UNIVERSITY, continued:

Research Assistant II 2001-2004

Oversaw laboratory functions, including maintaining equipment sterilization, monitoring user procedures to ensure contamination-free environment, and collecting specifically selected cell types for tissue culture-based experiments. Managed animal colony used in research / testing. Controlled inventory, stock, and reagents. Conducted machinery maintenance, hazardous waste documentation, and personnel training in proper laboratory procedure.

• Increased yield of specific developmental subsets of cells by 40% by creating unique strategy for isolating subsets of B lymphocytes from mice used in cellular signaling-controlled B cell experiments.

• Maintained detailed records of all reagents used across 3 post-doctoral fellow and 3 graduate students by designing and implementing a collaborative work environment that encouraged reagent and isolated cell sharing.

VOLUNTEER STATE COMMUNITY COLLEGE, Gallatin, Tennessee 2012

Public educational facility providing academic services to 8K students across 12 counties.

Adjunct Instructor

Coordinated lectures and learning activities for weekly courses, providing online support and developing additional material based on textbook packages. Led parallel laboratory didactic and practicum experimental coursework. Created exams, developed study guides, offered office hour support, and evaluated student progress, adjusting information delivery to ensure positive outcomes across various learning styles.

• Achieved target learning objectives by using numerous tools that enabled undergraduates without significant science experience to understand and absorb complex scientific terms and abilities.

• Developed communication, public speaking, and leadership skills by guiding students to successful course completion.

EDUCATION

Doctor of Philosophy in Cellular and Molecular Pathology (2008)

Vanderbilt University, Nashville, Tennessee

Bachelor of Science in Biology  (2001)

University of Arkansas, Fayetteville, Arkansas

TECHNICAL SKILLS

Microsoft Office (Word  Excel  PowerPoint)

PROFESSIONAL MEMBERSHIPS

Heart Rhythm Society  American Heart Association  American Association for the Advancement of Science

PUBLICATIONS

1. Lowe JS, Stroud DM, Yang T, Hall L, Atack TC, Roden DM.Increased late sodium current contributes to long QT-related arrhythmia susceptibility in female mice. Cardiovascular Res. 2012 Aug 1;95(3):300-7. doi: 10.1093/cvr/cvs160. Epub 2012 May 4.PubMed [citation] PMID: 22562703, PMCID: PMC3633400

2. Watanabe H, Yang T, Stroud DM, Lowe JS, Harris L, Atack TC, Wang DW, Hipkens SB, Leake B, Hall L, Kupershmidt S, Chopra N, Magnuson MA, Tanabe N, Knollmann BC, George AL Jr, Roden DM.Striking In vivo phenotype of a disease-associated human SCN5A mutation producing minimal changes in vitro. Circulation. 2011 Aug 30;124(9):1001-11. doi: 10.1161/CIRCULATIONAHA.110.987248. Epub 2011 Aug 8.PubMed [citation] PMID: 21824921, PMCID: PMC3297976

3. Atack TC, Stroud DM, Watanabe H, Yang T, Hall L, Hipkens SB, Lowe JS, Leake B, Magnuson MA, Yang P, Roden DM.Informatic and functional approaches to identifying a regulatory region for the cardiac sodium channel. Circ Res. 2011 Jun 24;109(1):38-46. doi: 10.1161/CIRCRESAHA.110.235630. Epub 2011 May 12.PubMed [citation] PMID: 21566215, PMCID: PMC3135383

4. Singh MV, Kapoun A, Higgins L, Kutschke W, Thurman JM, Zhang R, Singh M, Yang J,  Guan X, Lowe JS, Weiss RM, Zimmermann K, Yull FE, Blackwell TS, Mohler PJ, Anderson ME.Ca2+/calmodulin-dependent kinase II triggers cell membrane injury by  inducing complement factor B gene expression in the mouse heart.J Clin Invest. 2009 Apr;119(4):986-96. doi: 10.1172/JCI35814. Epub 2009 Mar 9.PubMed [citation]  PMID: 19273909, PMCID: PMC2662543

5. Kline CF, Cunha SR, Lowe JS, Hund TJ, Mohler PJ.Revisiting ankyrin-InsP3 receptor interactions: ankyrin-B associates with the cytoplasmic N-terminus of the InsP3 receptor. J Cell Biochem. 2008 Jul 1;104(4):1244-53. doi: 10.1002/jcb.21704.PubMed [citation] PMID: 18275062, PMCID: PMC2858327

6. Erickson JR, Joiner ML, Guan X, Kutschke W, Yang J, Oddis CV, Bartlett RK, Lowe JS, O'Donnell SE, Aykin-Burns N, Zimmerman MC, Zimmerman K, Ham AJ, Weiss RM, Spitz DR, Shea MA, Colbran RJ, Mohler PJ, Anderson ME.A dynamic pathway for calcium-independent activation of CaMKII by methionine oxidation. Cell. 2008 May 2;133(3):462-74. doi: 10.1016/j.cell.2008.02.048.PubMed [citation] PMID: 18455987, PMCID: PMC2435269

7. Lowe JS, Palygin O, Bhasin N, Hund TJ, Boyden PA, Shibata E, Anderson ME, Mohler PJ.Voltage-gated Nav channel targeting in the heart requires an ankyrin-G dependent cellular pathway. J Cell Biol. 2008 Jan 14;180(1):173-86. doi: 10.1083/jcb.200710107. Epub 2008 Jan 7.PubMed [citation] PMID: 18180363, PMCID: PMC2213608

8. Mohler PJ, Le Scouarnec S, Denjoy I, Lowe JS, Guicheney P, Caron L, Driskell IM, Schott JJ, Norris K, Leenhardt A, Kim RB, Escande D, Roden DM.Defining the cellular phenotype of "ankyrin-B syndrome" variants: human ANK2 variants associated with clinical phenotypes display a spectrum of activities in cardiomyocytes. Circulation. 2007 Jan 30;115(4):432-41. Epub 2007 Jan 22.PubMed [citation] PMID: 17242276

9. Hoek KL, Antony P, Lowe J, Shinners N, Sarmah B, Wente SR, Wang D, Gerstein RM, Khan WN.Transitional B cell fate is associated with developmental stage-specific regulation of diacylglycerol and calcium signaling upon B cell receptor engagement. J Immunol. 2006 Oct 15;177(8):5405-13.PubMed [citation] PMID: 17015726

10. Mohler PJ, Lowe JS, Banks S. Dysfunction in ankyrin-based cellular pathways and human cardiac arrhythmia. Future Cardiol. 2005 May;1(3):363-71. doi: 10.1517/14796678.1.3.363.PubMed [citation] PMID: 19804119

11. Antony P, Petro JB, Carlesso G, Shinners NP, Lowe J, Khan WN.B-cell antigen receptor activates transcription factors NFAT (nuclear factor of activated T-cells) and NF-kappaB (nuclear factor kappaB) via a mechanism that involves diacylglycerol. Biochem Soc Trans. 2004 Feb;32(Pt 1):113-5.PubMed [citation] PMID:

14748726

12. Antony P, Petro JB, Carlesso G, Shinners NP, Lowe J, Khan WN.B cell receptor directs the activation of NFAT and NF-kappaB via distinct molecular mechanisms. Exp Cell Res. 2003 Nov 15;291(1):11-24.PubMed [citation] PMID: 14597404

13. Petro JB, Gerstein RM, Lowe J, Carter RS, Shinners N, Khan WN.Transitional type 1 and 2 B lymphocyte subsets are differentially responsive to antigen receptor signaling. J Biol Chem. 2002 Dec 13;277(50):48009-19. Epub 2002 Sep 28.PubMed [citation] PMID: 12356763