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Brian Strahl, Ph.D. : Curriculum Vitae
Brian Strahl - Education and Research Experience
2014-present Professor, Department of Biochemistry and Biophysics, University of North Carolina School of Medicine
2010-present Faculty Director of the High-Throughput Peptide Synthesis and Arraying Facility, University of North Carolina School of Medicine
2008-present Associate Professor, Department of Biochemistry and Biophysics, University of North Carolina School of Medicine
2003-present Member of the UNC Lineberger Comprehensive Cancer Center
2002-2008 Assistant Professor, Department of Biochemistry and Biophysics, University of North Carolina School of Medicine
1998-2002 Postdoctoral Fellow, Department of Biochemistry and Molecular Genetics, University of Virginia. Mentor: Dr. C. David Allis
1993-1998 Ph.D. in Biochemistry. Department of Biochemistry, North Carolina State University. Mentor: Dr. William L. Miller
1988-1993 B.A. in both Chemistry and Biology. University of North Carolina at Greensboro. Mentor: Dr. Julian Lombardi
Honors and Awards
2009 Recipient of the Ruth and Phillip Hettleman Prize for Artistic and Scholarly Achievement, UNC
2006 Named as a Jefferson-Pilot Fellow in Academic Medicine, UNC
2006 Recipient of the University of North Carolina at Greensboro Young Alumni Award
2005 Recipient of the North Carolina State University Outstanding Alumnus Award
2004 Pew Scholar (Pew Scholars Program in the Biomedical Sciences)
2003 Recipient of a Presidential Early Career Award for Scientists and Engineers (PECASE)
2002 Recipient of a UNC Research Council award
1999-2002 National Institute of Health postdoctoral NRSA fellowship award
1998 Recipient of the Becton-Dickinson award for outstanding research in Biochemistry (from Ph.D. thesis)
1993 Recipient of the American Institute of Chemist Foundation award for being an outstanding senior student majoring in chemistry.
1992 Scholarship award by Special Support Services for outstanding scholarship throughout the year
1992 Member of the ßßß Biology Honors Society
Other Professional Experience
2015-present Editorial board member of Journal of Biological Chemistry
2014 Ad-hoc reviewer for Genes, Genomes, and Genetics (GGG) study section (Special Emphasis Panel ZRG1 GGG Q), NIH
2013-present Editorial board member of Epigenetics & Chromatin
2012 Ad-hoc reviewer for the NIH transformative research award initiative (Special Emphasis Panel ZRG1 BCMB-A), NIH
2012 Ad-hoc reviewer for theNIH Director’s Early Independence Award (Special Emphasis PanelZRG1 BBBP-E), NIH
2009-present Editorial board member of Molecular and Cellular Biology
2008 Ad-hoc reviewer; Fungal Genetics Special Emphasis Panel, NIH
2006 Ad-hoc review panel member for NIDA Study Section, NIH
2005 Ad-hoc review panel member for MG-C Study Section, NIH
Publications
Undergraduate:
1. Strahl, B. D. & Lombardi, J. (1994). Microdetermination of dry mass content in the uterine fluid of four species of viviparous sharks (Squalus acanthias, Carcharhinus plumbeus, Mustelus canis and Rhizoprionodon terraenovae). Comp. Biochem. Physiol. 108A:213-219.
Graduate:
2. Ghosh, B. R., Wu, J. C., Strahl, B. D., Childs, G. V. & Miller, W. L. (1996). Inhibin and estradiol alter gonadotropes differentially in ovine pituitary cultures: changing gonadotrope numbers and calcium responses to gonadotropin-releasing hormone. Endocrinology 137:5144-5154.
3. Strahl, B. D., Huang, H.-J., Pederson, N. R., Wu, J. C., Gosh, B. R. & Miller, W.L. (1997). Two proximal AP-1-binding sites are sufficient to stimulate transcription of the ovine follicle-stimulating hormone-ß gene. Endocrinology 138:2621-2631.
4.Strahl, B. D., Huang, H.-J., Sebastian, J., Ghosh, B. R. & Miller, W. L. (1998). Transcriptional activation of the ovine follicle-stimulating hormone ß-subunit gene by GnRH: involvement of two AP-1-binding sites and protein kinase C. Endocrinology 139:4455-4465.
5. Huang, H-J., Sebastian J., Strahl, B. D., Wu, J. C. & Miller, W. L. (2001). The promoter for ovine follicle-stimulating hormone-ß gene (FSHß) confers FSHß-like expression on luciferase in transgenic mice: regulatory studies in vivo and in vitro. Endocrinology 142:2260-2266.
6.Huang, H-J., Sebastian J., Strahl, B. D., Wu, J. C. & Miller, W. L. (2001). Transcriptional Regulation of the ovine FSH-beta gene by activin and GnRH: Involvement of two proximal AP-1 Sites for GnRH-Stimulation. Endocrinology 142:2267-2274.
7. Miller, W. L., Shafiee-Kermani, F., Strahl B. D., Huang, H. J. (2002) The nature of FSH induction by GnRH. Trends Endocrinol. Metab. 6:257-263.
Postdoctoral:
8.Strahl, B. D., Ohba, R., Cook, R. G. & Allis, C. D. (1999). Methylation of histone H3 at lysine 4 is highly conserved and correlates with transcriptionally active nuclei in Tetrahymena. Proc. Natl. Acad. Sci. USA 96:14967-14972.
9. Strahl, B. D. & Allis, C. D. (2000). The language of covalent histone modifications. Nature 403:41-45.
10. Rea, S., Eisenhaber, F., O’Carroll, D., Strahl, B. D., Sun, Z-W, Opravil, S., Schmid, M., Mechtler, K., Ponting, C., Allis, C. D. & Jenuwein, T. (2000). Regulation of chromatin structure by site-specific histone H3 methyltransferases. Nature 406:593-599 (Nature article).
11. Nakayama, J. -I., Rice J. C., Strahl, B. D., Allis, C. D. & Grewal, S. I. S. (2001) Role of histone H3 lysine 9 methylation in epigenetic control of heterochromatin assembly. Science 292:110-113.
12. Strahl, B. D.*, Briggs, S. D.*, Brame, C. J., Caldwell, J. A. Koh, S., Ma, H., Cook, R. G., Shabanowitz, J., Hunt D. F., Stallcup, M. R. & Allis, C. D. (2001) Methylation of histone H4 at arginine 3 occurs in vivo and is mediated by the nuclear receptor coactivator PRMT1. Current Bio. 11:996-1000.
13. Wang, H, Huang, Z. -Q., Xia, l., Feng, Q., Erdjument-Bromage, H., Strahl, B. D., Briggs, S. D. Allis C. D, Wong, J., Tempst, P. & Zhang, Y. (2001) Methylation of histone H4 at arginine 3 facilitates transcriptional activation by nuclear hormone receptor. Science 293:853-857.
14. Briggs, S. D., Bryk, M. Strahl, B. D., Cheung, W. L. Davie, J. K., Dent, Y. R. S., Winston, F. & Allis, C. D. (2001) Histone H3 lysine 4 methylation is mediated by Set1 and required for rDNA silencing in Saccharomyces cerevisiae. Genes and Dev. 15:3286-3295.
15. Bryk, M., Briggs, S. D., Strahl B. D., Curcio, M. J., Allis, C. D. & Winston F. (2002) Set1, a factor required for methylation of histone H3, regulates rDNA silencing in Saccaromyces cervisiae by a Sir2-independent mechanism. Current Bio. 12:165-170.
16. Ma, H., Baumann, C. T., Li, H., Strahl, B.D., Rice, R., Jelinek, M. A., Aswad, D. W., Allis, C. D., Hager, G. L. & Stallcup, M. R. (2001) Hormone-dependent, CARM1-directed, arginine-specific methylation of histone H3 on the mouse mammary tumor virus promoter. Current Bio. 11:1981-1985.
17. Strahl, B. D., Grant, P. A., Briggs, S. D., Bone J. R., Caldwell, J. A., Cook, R. G., Sun, Z.-W., Mollah, S., Shabanowitz, J., Hunt, D. F. & Allis, C. D.(2002)Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol. Cell. Biol. 22:1298-1306.
18. Li, J., Lin, Q., Yoon, H.-G., Strahl, B. D., Allis, C. D. & Wong, J. (2002) Involvement of histone methylation and phoshporylation in regulation of transcription by thyroid hormone receptor. Mol. Cell. Biol. 22:5688-5697.
UNC-CH:
19. Briggs, S. B. & Strahl, B. D. (2002) Unraveling heterochromatin. Nature Genet., 30:241-242.
20. Briggs, S. B., Xiao, T., Sun, Z.-W., Caldwell, J. A., Shabanowitz, J., Hunt, D. F., Allis, C. D. & Strahl, B. D. (2002)Trans-histone regulatory pathway in chromatin. Nature, 418:498.
21. Xiao, T., Hall, H., Kizer, K. O., Shibata, Y., Hall, M. C., Borchers, C. H. & Strahl, B. D. (2003) Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. Genes & Dev. 17:654-663.
22. Anest, V., HansonJ. L., CogswellP. C., SteinbrecherK. A., StrahlB. D., & BaldwinA. S. (2003) A nucleosomal function for IkB kinase-b in NF-kB-dependent gene expression. Nature, 423:659-663.
23. Lee, C.-K., Shibata, Y., Rao, B., Strahl, B. D. & Lieb, J. D. (2004) Evidence for Nucleosome Depletion at Active Regulatory Regions Genomewide. Nature Genet., 36:900-905.
24. Lee, D. Y., Teyssier, C., Strahl, B. D. & Stallcup, M. R. (2005) Role of Protein Methylation in Regulation of Transcription. Endocrine Reviews 26:147-170.
25. Xiao, T. Kao, C. F., Krogan, N., Sun, Z.-W., Greenblatt, J. F., Osley, M. A., & Strahl, B. D. (2005) Histone H2B ubiquitylation is associated with elongating RNA polymerase II. Mol Cell Biol. 25:637-651.
26. Kizer, O. K., Phatnani, H. P., Shibata, Y., Hall, H., Greenleaf, A. L. & Strahl, B. D. (2005) A novel domain in Set2 mediates RNA polymerase II interaction and couples histone H3 K36 methylation with transcription elongation. Mol Cell Biol. 25:3305-3316.
27. Adhvaryu, K. K., Morris, S., Strahl, B. D. & Selker, E.U. (2005) Methylation of histone H3 lysine 36 is required for normal development in Neurospora crassa. Eukaryot Cell. 4:1455-1464.
28. Morris, S. A., Shibata, Y., Noma, K.-I., Tsukamoto, Y., Warren, E., Temple, B., Grewal, S. I. S. & Strahl, B. D. (2005) Histone H3 K36 methylation is associated with transcription elongation in Schizosaccharomyces pombe. Eukaryot Cell. 4:1446-1454.
29. Laribee, R. N., Krogan, J. N., Xiao, T., Shibata, Y., Hughes, T. R., Greenblatt, J. F. & Strahl, B. D. (2005) BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex. Current Bio. 15:1487-1493.
30. Rao, B., Shibata, Y., Strahl, B. D. & Lieb, J. D. (2005) Dimethylation of Histone H3 at Lysine 36 Occurs Co-transcriptionally to Demarcate Regulatory and Non-Regulatory Chromatin Genome-wide. Mol Cell Biol. 25:9447-9459.
31. Keogh, M.-C., Kurdistani, S. K., Morris, S. A., Ahn, S. H., Collins, S. R., Podolny, V., Chin, K., Punna, T., Thompson, N. J., Boone, C., Emili, A., Weissman, J. S., Hughes, T. R., Strahl, B. D., Grunstein, M., Greenblatt, J. F., Buratowski, S., & Krogan, N. J. (2005) Co-transcriptional Set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. Cell 123:593-605.
32. Vojnic, E., Simon, B., Strahl, B. D., Sattler, M. & Cramer, P. (2006) Structure and CTD binding of the Set2 SRI domain that couples histone H3 K36 methylation to transcription. J Biol Chem. 281:13-15.
33. Tripic, T., Edmondson, D., Davie, J, Strahl, B. D. & Dent, S.R. (2006) The Set2 methyltransferase associates with Ssn6 yet Tup1-Ssn6 repression is independent of histone methylation. Biochem Biophys Res Comm. 339:905-14.
34. Biswas, D., Duttu-Biswas, R., Mitra, D., Shibata, Y., Strahl, B. D., Formosa, T., Stillman, D. J. (2006) Opposing roles for Set2 and yFACT in regulating TBP binding at promoters. EMBO J. 25:4479-4489.
35. Kizer, O. K., Xiao, T. & Strahl, B. D. (2006)Accelerated nuclei preparation and methods for the analysis of histone modifications in yeast. Methods 40:296-302.
36. Xiao, T., Shibata, Y., Rao, B., Laribee, R. N., Krogan, J. N., Greenblatt, J. F., Rourke, R. O., Buck, M. J., Lieb, J. D. & Strahl, B. D. (2007) The RNA Pol II kinase Ctk1 regulates positioning of a 5' histone methylation boundary along genes. Mol Cell Biol. 27:721-731.
37. Garcia, B. A., Hake, S. B., Diaz, R. L., Kauer, M., Morris, S. A., Recht, J., Shabanowitz, J., Mishra, N., Strahl, B. D., Allis, C. D. & Hunt, D. F. (2007) Organismal differences in post-translational modification in histones H3 and H4. J Biol Chem. 282:7641-7655.
38. Morris,S. A., Rao,B., Garcia,B. A., Hake,S. B., Diaz,R. L., Shabanowitz,J., Hunt,D. F., Allis,C. D., Lieb, J. D. & Strahl, B. D. (2007) Identification of histone H3 lysine 36 acetylation as a highly conserved modification. J Biol Chem. 282:7632-7640.
39. Laribee, R. N., Fuchs, S. M. & Strahl B. D. (2007) H2B ubiquitylation in transcriptional control: a FACT finding mission. Genes & Dev. 21:737-743.
40. Laribee, R. N., Shibata, Y., Mersman, D. P., Roguev, A.,Collins, S. R., Kemmeren, P., Weissman, J. S., Briggs, S. D., Krogan, N. J.* & Strahl, B. D.* (2007).The CCR4/NOT complex associates with the proteasome and regulates histone methylation. Proc Natl Acad Sci USA 104:5836-5841.
41. Wyce, A., Xiao, T., Whelan, K. A., Kosman, C., Walter, W., Eick, D., Hughes, T. R., Krogan, N. J., Strahl, B. D. & Berger, S. L. (2007) H2B ubiquitylation acts as a barrier to Ctk1 nucleosomal recruitment prior to removal by Ubp8 within a SAGA-related complex. Mol Cell. 27:275-88.
42. Rivenbark, A. G. & Strahl B. D. (2007) Unlocking cell fate. Science 318:403-404.
43. Merker, J. D., Dominska, M., Greenwell, P. W., Rinella, E., Bouck, D. C., Shibata, Y., Strahl, B. D., Mieczkowski, P. & Petes, T. D. (2008) The histone methylase Set2p and the histone deacetylase Rpd3p repress meiotic recombination at the HIS4 meiotic recombination hotspot in Saccharomyces cerevisiae. DNA Repair 7:1298-308.
44. Youdell, M. J.*, Kizer, O. K.*, Kisseleva-Romanova, E. Fuchs, S. M., Duro, E., Korn, K., Strahl,B. D. & Mellor, J. (2008) Spt6 controls methylation of lysine 36 on histone H3 to stabilize transcribed chromatin. Mol Cell Biol. 16:4915-4926.
45. Henikoff, S., Strahl, B. D. & Warburton P. E. (2008) Epigenomics: a roadmap to chromatin. Science. 322:853.
46. Fuchs, S. M., Laribee, R. N. & Strahl, B. D. (2009) Protein modifications in transcription elongation. BBA - Gene Regulatory Mechanisms. 1789:26-36.
47. Lickwar, C.*, Rao, B.*, Shabalin, A., Nobel, A., Strahl, B. D. & Lieb, J. D. (2009) The Set2/Rpd3S pathway suppresses cryptic transcription without regard to gene length or transcription frequency PLoS ONE. 4:e4886
48. Nakanishi, S., Lee, J. S., Gardner, K. E., Gardner, J. M., Takahashi, Y-H., Chandrasekharan, M. B., Sun, Z-W., Osley, M. A., Strahl, B. D., Jaspersen, S. L. & Shilatifard, A. (2009) Histone H2BK123 monoubiquitination is the critical determinant for H3K4 and H3K79 trimethylation by COMPASS and Dot1. J of Cell Biol. 186:371-377.
49. Fuchs, S. M., Krajewski, K., Miller, V., Baker, R. W. & Strahl, B. D. (2011) Influence of combinatorial histone modifications on antibody and effector protein recognition. Current Biology. 11:53-58.
50. Ramachandran, S., Vogel, L., Strahl, B. D.* & Dokholyan, N. V.* (2011) Thermodynamic stability of protein-protein interaction is a necessary but not sufficient driving force for evolutionary conservation. PLoS Comput Biol. 7:e1001042.
51. Gardner K. E., Zhou, L., Parra, M. A., Chen, X. & Strahl, B. D. (2011)Identification of lysine 37 of histone H2B as a novel site of methylation. PLoS ONE. 6:e16244.
52. Gardner, K. E., Allis, C. D. & Strahl, B. D. (2011) OPERating ON chromatin, a colorful language where context matters. J. Mol. Biol. 409:36-46.
53. Kerr, S. C., Azzouz, N., Fuchs, F. S., Collart, M. A., Strahl, B. D., Corbett, A. H. & Laribee, R. N. (2011) The CCR4-NOT complex physically and functionally interacts with the mRNA export pathway. PLoS ONE. 6:e18302.
54. Fuchs, S. M. & Strahl, B. D. (2011) Antibody recognition of histone post-translational modifications: emerging issues and future prospects. Epigenomics. 3:247-249.
55. Fuchs, S. M., Kizer, K. O., Braberg, H., Krogan, N. & Strahl, B. D. (2012)RNA polymerase II CTD phosphorylation regulates protein stability of the Set2 methyltransferase and histone H3 di- and trimethylation at lysine 36. J Biol Chem. 287:3249-3256.
56. Rivenbark, A. G., Stolzenburg, S., Yuan, X., Strahl, B. D. & Blancafort, P. (2012) Epigenetic reprogramming of cancer cells by targeted DNA methylation. Epigenetics. 7:1-11.
57. Rothbart, S. B., Lin, S., Britton, L.-M., Krajewski, K., Keogh, M.-C., Garcia, B. & Strahl, B. D. (2012) Poly-acetylated chromatin signatures are preferred epitopes for site-specific histone H4 acetyl antibodies. Scientific Reports. 2:489.
58. Rothbart, S. B., Krajewski, K., Strahl B. D., Fuchs, S. M. (2012) Peptide microarrays to interrogate the histone code. Methods Enzymol. 512:107-135.
59. Stolzenburg, S., Rots, M. G., Beltran, A. S., Rivenbark, A. G., Yuan, X., Strahl, B. D. & Blancafort, P. (2012) Targeted silencing of the oncogene transcription factor SOX2 in breast cancer. Nucleic Acid Res. 40:6725-6740.
60. Bánfai, B., Jia, H., Khatun, J., Wood, E., Risk, B., Gundling, W., Kundaje, A., Gunawardena, H. P., Yu, Y., Xie, L., Krajewski, K., Strahl, B. D., Chen, X., Bickel, P. J., Giddings, M. C., Brown, J. B. & Lipovich, L. (2012) Long non-coding RNAs are rarely translated. Genome Research. 22:1646-1657.
61. Rothbart, S. B., Krajewski, K., Nady, N., Tempel, W., Xue, S., Badeaux, A. I., Barsyty-Lovejoy, D., Martinez, J. Y., Bedford, M. T., Fuchs, S. M., Arrowsmith, C. H. & Strahl, B. D. (2012) Association of UHRF1 with methylated H3K9 directs the maintenance of DNA methylation. Nature Structural & Molecular Biology. 19:1155-1162.
· News & Views article highlighting this work was featured in Epigenomics (2012) 4:597-599.
62. Rizzardi, L. F., Dorn, E. E. Strahl B. D. & Cook J. G. (2012) H3K4 di-methylation promotes DNA replication origin function in Saccharomyces cerevisiae. Genetics 192:371-384.
63. Nishikori, S., Fuchs, S. M., Yasui, N., Wojcik, J., Koide, A., Strahl, B. D. & Koide, S. A (2012) quantitative and sensitive method for characterizing anti-histone antibodies. J. Mol. Biol. 424:391-399.
64. Ali, M., Yan, K., Lalonde, M.-E., Degerny, C., Rothbart, S. B., Strahl, B. D., Cote, J., Yang, X-.J. & Kutateladze, T., G. (2012) Tandem PHD fingers of MORF/MOZ acetyltransferases display selectivity for acetylated histone H3 and are required for the association with chromatin. J. Mol. Biol. 424:328-288.
65. Cai, L., Rothbart, S. B., Lu, R., Xu, B., Tripathy, A., Chen, W.-Y., Zheng, D., Patel, D. J., Allis, C. D., Strahl, B. D., Song, J., Wang, G. G. (2013) An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting. Molecular Cell. 49:571-582.
66. Law, J. A., Du, J., Hale, C. J., Feng, S., Krajewski, K., Strahl, B. D., Patel, D. J. & Jacobsen, S. E. (2013) SHH1 recruits RNA Polymerase-IV to RNA-directed DNA methylation targets. Nature. 498:385-389.
67. Rothbart, S. B., Dickson, B. M., Ong, M. S., Krajewski, K., Houliston, S., Kireev, D. B., Arrowsmith, C. H. & Strahl, B. D. (2013) Multivalent histone engagement by the linked tandem Tudor and PHD domains of UHRF1 is required for the epigenetic inheritance of DNA methylation. Genes & Development. 27:1288-1298.
68. Ali, M., Rincon-Arano, H., Zhao., W., Rothbart, S. B., Tong, Q., Parkhurst, S., Strahl B. D., Deng, L.-W., Groudine, M., Kutateladze, T. G. (2013) Molecular basis for chromatin binding and regulation of MLL5. Proc Natl Acad Sci USA. 110:11296-11301.
69. Gatchalian, J., Fütterer, A., Rothbart, S. B.,Tong, Q., Rincon-Arano, H., Sánchez de Diego, A. Groudine, M., Strahl, B. D., Martínez-A, C., van Wely, H. M. K. & Kutateladze, T. G.Dido3 PHD modulates cell differentiation and division. (2013) Cell Reports. 11:148-158.
70. Kinkelin, K., Wozniak, G. G., Rothbart, S. B., Lidschreiber, M., Strahl B. D. & Cramer, P. Structures of RNA polymerase II complexes with Bye1, a chromatin-binding PHF3/DIDO1 homologue. (2013) Proc Natl Acad Sci USA. 110:15277-15282.
71. McDaniel S. L. & Strahl, B. D. (2013) Stress-Free with Rpd3: A unique chromatin complex mediates the response to oxidative stress. Mol Cell Biol. 33:3726-3727.
72. Hattori, T., Taft, J., Swift, K., Luo, H., Witt, H., Slattery, M., Koide, A., Ruthenburg, A. J., Krajewski, K., Strahl, B. D., White, K. P., Farnham, P. J., Zhao, Y., Koide, S. (2013) Recombinant antibodies to histone posttranslational modifications. Nature Methods. 10:992-995.
73. Dronamraju, R. & Strahl B. D. (2014) A feed forward circuit comprising Spt6, Ctk1 and PAF regulates Pol II CTD phosphorylation and transcription elongation. Nucleic Acids Research.42(2):870-881.
74. Klein, B. J., Piao, L., Xi, Yuanxin, Rincon-Arano, H., ROthbart, S. B., Larson, C., Wen, H., Zheng, X., Cortazar, M., Pena, P. V., Mangan, A., Bentley, D. L., Strahl, B. D., Groudine, M., Li, W., Shi, X., Kutateladze, T. G. (2014) The histone-H3K4-specific demethylase KDM5B binds to its substrate and product through distinct PHD dingers. Cell Reports. 6:1-11.
75. Kim, H.-S., Mukhopadhyay, R., Rothbart, S. B., Silva, A. C., Vanoosthuyse, V., Radovani, E., Kislinger, T., Roguev, A., Ryan, C. J., Xu, J., Jahari, H., Hardwick, K., G., Greenblatt, J. F., Krogan, N. J., Fillingham, J., S., Strahl, B. D., Bouhassira, E., E., Edelmann, W. & Keogh, M.-C. (2014) Identification of a novel Bromodomain/Casein Kinase II/TAF-containing complex as a regulator of mitotic condensin function. Cell Reports. 6:1-14
76. Rothbart S. B. & Strahl, B. D. (2014) Interpreting the language of histone and DNA modifications. BBA - Gene Regulatory Mechanisms. 10.1016/j.bbagrm.2014.03.001
77. Wozniak, G. G. & Strahl, B. D. (2014) Hitting the 'Mark': Interpreting Lysine Methylation in the Context of Active Transcription. BBA - Gene Regulatory Mechanisms. 10.1016/j.bbagrm.2014.03.002.
78. Greer, E. L., Beese-Sims, S. E., Spadafora, R., Rothbart, S. B., Badeaux, A. I., Strahl, B. D., Colaiácovo, M. P. & Shi, Y. A (2014) histone methylation network regulates transgenerational epigenetic memory in C. elegans. Cell Reports. 7:113-26.
79. Jha, D. K. & Strahl, B. D. (2014) H3K36 methylation regulates chromatin remodeling and checkpoint activation after DSB. Nature Commun. 5:3965.
80. Shanle, E. K., Rothbart, S. B. & Strahl, B. D. (2014) Chromatin biochemistry enters the next generation of code ‘seq-ing’ Nature Methods. 11:799-800.
81. Wozniak, G. G. & Strahl, B. D. (2014) Catalysis-dependent stabilization of Bre1 fine-tunes histone H2B ubiquitylation to regulate gene transcription. Genes and Development. 28:1647-1652.
82. Jha, D. K. & Strahl, B. D. (2014) SET-ing the stage for DNA repair. Nat Struct Mol Biol. 5:655-657.
83. Gilbert, T. M.*, McDaniel, S. L.*, Byrum., S. D., Cades, J. A., Dancy, B. C. Y, Wade, H., Tackett, A. J., Strahl, B. D.* & Taverna, S. D.* (2014)A PWWP domain-containing protein targets the NuA3 acetyltransferase complex via H3 lysine 36 trimethylation to coordinate transcriptional elongation at coding regions. Mol Cell Proteomics. 13:2883-2895.
84. Dumesic, P. A., Homer, C. M., Moresco, J. J., Pack, L. R., Shanle, E. K., Coyle, S. M., Strahl, B. D., Fujimori, D. G., Yates, J. R. & Madhani, H. D. (2015) Product binding enforces the genomic specificity of a yeast polycomb repressive complex. Cell. 160:204-218.
85. Tong, Q., Cui, G., Botuyan, M. V., Rothbart, S. B., Hayashi, R., Musselman, C. A., Appella, E., Strahl, B. D., Mer, G. & Kutateladze, T. G. (2015) Structural plasticity of methyllysine recognition by the tandem Tudor domain of 53BP1. Structure.23:312-21.
86. Tong, Q., Mazur, S., J., Rincon-Arona, H., Rothbart, S. B., Kuznetsov, D. M., Cui, G., Liu, W. H., Gete, Y., Klein, B. J., Jenkins, L., Mer, G., Kutatelasze, A., G., Strahl, B. D., Groudine, M., G., Appella, E. & Kutateladze, T., G. An acetyl-methyl switch drives a conformational change in p53. Structure.23:322-31.
87. McKay, D. J., Klusza, S., Penke, T. J., R., Meers, M. P., Curry, K. P., McDaniel, S. L., Malek, P. Y., Cooper, S. W., Tatomer, D., C., Lieb, J. D., Strahl, B. D., Duronio, R. J. & Matera, A. G. (2015) Interrogating the function of metazoan histones using engineered gene clusters. Developmental Cell.32:373-86.
88. Perfetti, M. T., Baughman, B. M., Dickson, B. D. Mu, Y., Cui, G., Mader, P., Dong, A., Norris, J. L., Rothbart, S. B., Strahl, B. D., Brown, P. J., Janzen, W. P., Arrowsmith, C. H., Mer, G., McBride, K., James, L., & Frye, S. V. (2015) Identification of a Fragment-like Small Molecule Ligand for the Methyl-lysine Binding Protein, 53BP1. ACS Chemical Biology. 10:1072-1081.
89. Rothbart, S. B., Dickson, B. M. & Strahl, B. D. (2015) From Histones to Ribosomes: A Chromatin Regulator Tangoes with Translation. Cancer Discovery. 5:228-30.
90. Wang, L., Xie, L., Ramachandran, S., Lee, Y., Yan, Z., Zhou, L., Krajewski, K., Liu, F., Zhu, C., Chen, D., Strahl, B. D., Jin, J., Dokholyan, N. V. & Chen, X. (2015) Non-canonical bromodomain within DNA-PKcs promotes DNA damage response and radioresistance through recognizing an IR-induced acetyl-lysine on H2AX. Chemistry & Biology.22:1-13.
91. Rothbart, S. B., Dickson, B. M., Raab, J. R., Grzybowski, A. T., Krajewski, K., Guo, A. H., Shanle, E. K., Josefowicz, S. Z., Fuchs, S. M., Allis, C. D., Magnuson, T. R., Ruthenburg, A. J. & Strahl, B. D. (2015) An interactive database for the assessment of histone antibody specificity. Molecular Cell. 59:502-511.
92. Ali, F. A., Daze, K. D., Rothbart, S. B., Strongin, D. E., Rincon-Arano, H., Allen, H. F., Li, J., Groudine, M., Strahl, B. D., Hof, F. & Kutateladze, T. G. (2015) Molecular Insights into Inhibition of the Methylated Histone-Plant Homeodomain Complexes by Calixarenes. Journal of Biological Chemistry. 290:22919-22930.
93. Zhang, Z.-M., Rothbart, S. B., Allison, D., Cai, Q., Harrison, J. S., Li, L., Wang, Y., Strahl B. D., Wang, G. G., Song, J. (2015) An allosteric interaction links USP7 to deubiquitination and chromatin targeting of UHRF1. Cell Reports. 12:1400-1406.
94. Simon, J. M., Parker, J. S., Liu, F., Rothbart, S. B., Ait-Si-Ali, S., Strahl, B. D., Jin, j., Davis, I. J., Mosley, A. L. & Pattenden, P. G. (2015) A role for Widely Interspaced Zinc finger (WIZ) in retention of the G9a methyltransferase on chromatin. Journal of Biological Chemistry. 290:26088-26102.
95. Shanle, E. K, Andrews, F. H., Meriesh, H., McDaniel, S. L., Dronamraiu, R., DiFiore J., Jha, D., Wozniak, G. G., Bridgers, J., Kerschner, J. L., Martín, G. M., Morrison A. J., Krajewski, K., Kutateladze, T.* & Strahl, B. D.* (2015) Association of Taf14 with acetylated histone H3 directs gene transcription and the DNA damage response. Genes & Development. 29:1789-1794.
96. Chen, S., Ze, Y., Wilkinson, A., Deshpande, A. J., Krajewski, K., Strahl, B. D., Armstrong, S. A., Patel, D., J. & Gozani, O. (2105) The PZP domain of AF10 senses unmodified H3K27 to regulate DOT1L-mediated methylation of H3K79. Molecular Cell.60:319-327.
97. Shanle, E. K., Tsun, I. K. & Strahl, B. D.(2015) A course-based undergraduate research experience investigating p300 bromodomain mutations. Biochemistry and Molecular Biology Education.doi: 10.1002/bmb.20927.
98. Cliffe, A. R., Vogel, J. L., Arbuckle, J. H., Geden, M. J., Rothbart, S. B., Cusack, C. L., Strahl, B. D., Kristie, T. M., Deshmukh, M. A (2015) Neuronal stress pathway mediating a histone Methyl/Phospho switch is required for Herpes Simplex Virus Reactivation. Cell Host and Microbe. 18:649-658.
99. Andrews, F. H.*, Gatchalian, J., Krajewski, K., Strahl, B. D. & Kutateladze, T. G. (2016) Regulation of methyllysine readers through phosphorylation. ACS Chemical Biology. DOI: 10.1021/acschembio.5b00802.
100. Andrews, F. H.*, Shanle, E. K.*, Strahl, B. D.* & Kutateladze, T. G.* (2016) Acetyllysine binding activity of the YEATS domains is essential for transcription. Transcription (In Press).
101. Hattori, T., Lai, D., Dementieva, I. S., Montano, S. P., Zheng, Y., Akin, L., Swift, K., M., Grzybowski, A. T., Koide, A., Krajewski, K., Strahl, B. D., Kelleher, N. L., Ruthenburg, A. J. & Koide, S. (2016) Antigen clasping by two antigen-binding sites of an exceptionally specific antibody for histone methylation. Proc Natl Acad Sci USA (In Press).
102. Cornett, E. M., Dickson, B. M., Vaughan, R. M., Trievel, R. C., Strahl, B. D. & Rothbart, S. B. Substrate specificity profiling of histone-modifying enzymes by peptide microarray. Methods in Enzymology. (In Press).
103. Sorenson, M. R., Jha, D. K., Ucles, S., Flood, D., Strahl, B. D., Stevens, S. W. & Kress, T. L.(2016)Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae. RNA Biology (In Press).
Extra-Mural Presentations
Invited Speaker:
7/14 FASEB Symposium on Biological Methylation: From DNA to Histones and Disease. Nassau, Bahamas.
5/14 Department of Biology, University of Oregon, OR
5/14 M.D. Anderson Cancer Center retreat, Keynote Speaker, TX
4/14 Massachusetts General Hospital Cancer Center, Harvard, MA
4/14 UNC LLC Cancer Center Symposium, NC
4/14 Department of Medical Biophysics, University of Toronto, CA
12/13 Department of Biochemistry, University of Southern California
12/13 Jonsson Comprehensive Cancer Center, UCLA
11/13 Department of Biological Chemistry, University of Michigan
9/13 Department of Molecular and Structural Biochemistry, North Carolina State University
9/13 Department of Biochemistry & Biophysics, University of North Carolina at Chapel Hill
5/13 University of Virginia School of Medicine Research Symposium on Epigenetics and Disease
5/13 Carolina Center for Genome Sciences Symposium, From Genome to Proteome: Proteomic Innovations and Technologies at UNC
4/13 Epigenetics & Chromatin: Interactions and processes, Harvard Medical School, Boston
4/13 Dept. of Molecular and Cellular Biochemistry, Ohio State University
10/12 ASBMB Special Symposium on Transcriptional Regulation by Chromatin and RNA Polymerase II, Snowbird Resort, UT
3/12 National Institute of Environmental Health Sciences, Research Triangle Park, NC
1/12 Department of Cancer Biology, Cleveland Clinic, Cleveland, OH
12/11 MD Anderson Cancer Center, Science Park at Smithville, TX
11/11 Department of Biochemistry & Cell Biology, Stony Brook University, Stony Brook, NY
10/11 Department of Molecular Biology and Genetics, Johns Hopkins University, MD.
10/11 David Allis 60th Birthday Symposium, Rockefeller, NY
9/11 BioTechniques Web Symposium – Understanding the role of Epigenetic change in gene regulation – Keynote Address (http://www.biotechniques.com/symposium)
7/11 FASEB Conference on Chromatin & Transcription, Snowmass, CO
6/11 Celgene Inc., San Diego, CA
3/11 Department of Genetics, Case Western University, OH
1/11 Keystone Symposium - Histone Code: Fact or Fiction, UT
11/10 Pew Scholars Reunion meeting, Duck Key, Fl
10/10 International Symposium on Animal Biology of Reproduction – Epigenetic Control of Reproductive Processes, Àuguas de São Pedro, Brazil
9/10 ASBMB Special Symposium on Transcriptional Regulation by Chromatin and RNA Polymerase II, Lake Tahoe, CA
5/10 FASEB Biological Methylation meeting, Carefree, Arizona
4/10 Hettleman award lecture, UNC-Chapel Hill, NC
4/10 Department of Biochemistry and Biophysics, Texas A&M, TX
3/10 UNC MD-PhD Program, UNC-School of Medicine, NC
3/10 Department of Biochemistry, Vanderbilt University, TN
11/09 National Institute of Environmental Health Sciences, Research Triangle Park, NC
5/09 Laval University Cancer Research Center, Quebec, Canada
12/08 Department of Genetics, UNC-Chapel Hill, NC
6/08 FASEB Conference on Transcriptional Regulation during Cell Growth, Differentiation, and Development, Snowmass, CO
6/08 FASEB meeting on Biological Methylation: From DNA to Histones,
Carefree, Arizona
4/08 Department of Biology, Georgia State University, GA
4/08 NIDA (NIH) workshop on the genetics and epigenetics of drug addiction, DC
3/08 Pew Scholars meeting, Aruba
11/07 Lankenau Institute for Medical Research, Wynnewood, PA
10/07 2nd annual Atlantic Coast Chromatin Conference, Chapel Hill, NC (speaker and co-organizer)
5/07 National Institute of Environmental Health Sciences, Research Triangle Park, NC
5/07 Department of Pathology, University of Iowa Health Care, IA
4/07 Keystone Symposium on Epigenetics: Regulation of Chromatin Structure in Development and Disease (session chair and invited speaker).
4/07 Department of Biochemistry, Michigan State University, MI
5/07 Department of Molecular Genetics, The Ohio State University, OH
12/06 Department of Molecular Oncology, McGill University, Canada
11/06 Fred Hutchinson Cancer Research Center, University of Washington
School of Medicine, Seattle, WA
10/06 Department of Biochemistry & Molecular Genetics, University of Virginia, VA
10/06 Department of Genetics, Yale University, New Haven, CT
10/06 Department of Pharmacology and Cancer Biology, Duke University, NC
9/06 Department of Biochemistry & Molecular, Biology Louisiana State University Health Sciences Center, LA
6/06 EuroConference on Gene Transcription in Yeast, San Feliu de Guixols, Spain
5/06 Gordon Research Conference on Chromatin Structure & Function, IL Ciocco, Italy (session chair and invited speaker)
4/06 Department of Chemistry, University of North Carolina at Greensboro, Greensboro, NC
4/06 ASBMB conference at San Francisco, CA (session chair and invited speaker)
1/06 15th Annual meeting of the Triangle Consortium for Reproductive Biology, Chapel Hill, NC
12/05 Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC
9/05 Department of Biochemistry and Molecular Biology, Southern Illinois University-School of Medicine, Carbondale, IL
9/05 Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, NY
5/05 National Institute of Drug Addiction Workshop on Epigenetics and
adaptation to drugs abuse, National Institutes of Health, DC
4/05 American Society of Biochemistry and Molecular Biology (ASBMB) Schering-Plough Awards Lecture, San Diego CA
3/05 Pew Scholars meeting, Cozumel, Mexico
1/05 Department of Molecular and Structural Biochemistry, North
Carolina State University
12/04 Department of Biochemistry, Emory University
11/04 Department of Chemistry, Wake Forrest University
8/04 Curriculum in Genetics and Molecular Biology Symposium at UNC
6/04 EuroConference on Gene Transcription in Yeast, San Feliu de Guixols, Spain
5/04 National Institute of Environmental Health Sciences, Research Triangle Park
4/04 28th Lineberger Cancer Center Symposium on "Epigenetics, Chromatin, and Cancer”
3/04 Interdisciplinary Program at the University of Florida
10/03 Banting and Best Department of Medical Research, University of Toronto
8/03 Cancer Cell Biology Program Retreat, University of North Carolina School of Medicine
5/03 Transcription Workshop, Marie Curie Research Institute, Oxted, Surrey, UK
5/03 H. Lee Moffitt Cancer Center, University of South Florida
4/03 Department of Genetics, North Carolina State University, NC
3/03 Keystone research conference on the enzymology of chromatin and transcription, Santa Fe, NM
9/02 Department of Biology, University of North Carolina at Greensboro, NC
2/02 National Institute of Environmental Health Sciences, Research Triangle Park, NC
7/01 FASEB research conference on biological methylation
Teaching Responsibilities
Present:
2003-present Genetics 632 “Advanced Molecular Biology II”, 12-13 lectures annually
2012-present Summer Course in Biophysics for Underrepresented Minorities, one lecture annually
Past:
2007-2012 Medical School’s “Molecules to Cells” course, one lecture annually
2006-2012 Biochemistry 715 “Scientific Oral Presentation”, one lecture annually plus managing/hosting weekly student seminar (co-course director)
2004 Biochemistry 221 “Cell Regulation by Ubiquitination”, one lecture
2002-2006 Ethics Course, group discussion leader, 2 hours annually
2008-2009 Group leader and advisor for first year BBSP graduate students (BBSP902)
2010 Summer Course in Biophysics: Case Studies in the Physics of Life, one lecture
2011-2012 Case Conference Leader for the “Molecules to Cells” Medical School course, one case conference annually
Society Memberships
2004 – present American Society for Microbiology (ASM)
2005 – present American Society of Biochemistry and Molecular Biology (ASBMB)
2013 – present American Association for the Advancement of Science (AAAS)
Journal Referee (current average of 4 papers/month)
BBA - Gene Regulatory Mechanisms, Cancer Cell, Current Biology, Cell, Gene Structure and Expression, EMBO Journal, EMBO Reports, Epigenetics & Chromatin, Eukaryotic Cell, FEBS Letters, Gene, Genetics, Genome Biology, Journal of Immunological Methods, Journal of Molecular Biology, Molecular and Cellular Biology, Molecular and Cellular Proteomics, Molecular Biology of the Cell, Molecular Cell, Methods in Enzymology, Nature, Nature Cell Biology, Nature Genetics, Nature Reviews Genetics, Nature Structural & Molecular Biology,Nucleic Acids Research, Nucleus, Oncogene, PLoS Biology, PLoS Genetics, PNAS, Science, Structure, Trends in Genetics
Extra-Mural Service
2003 Ad-hoc grant reviewer for NSF
2004 National Institute of Environmental Health Science faculty search committee
2005 National Institute of Environmental Health Science faculty search committee
2005 Task force member to evaluate the American Society of Biochemistry and Molecular Biology journal “ASBMB Today”
2006-present Co-organizer of the “Atlantic Coast Chromatin Research Club” to foster local interaction and communication between Research Triangle area chromatin researchers with monthly meetings and a yearly symposium
2007 National Institute of Environmental Health Science committee member for the evaluation and promotion of a senior postdoctoral researcher.
2012 Co-organizer of the 2012 FASEB Summer Research Conference on Biological Methylation
2012 National Institute of Environmental Health Science committee member for the evaluation and promotion of a senior postdoctoral researcher.
2012 Co-founder of EpiCypher, a new company centered on providing high quality and novel epigenetic reagents to the research community
2013 Ad-hoc grant reviewer for NSF
2013 Co-organizer of the “Carolina Chromatin Consortium (C3)” – a UNC-based chromatin and epigenetics chromatin interest group that meets monthly to foster local interaction and to stimulate synergistic collaborative research and grants within the UNC community.
2013 Scientific Advisory Board Member of RevMab, CA.
2014 Ad-hoc grant reviewer for NSF