ANAND PRABHAKAR

     Scientist,

     Biologics Development Centre,

     Dr. Reddy's Laboratories,

     Hyderabad (India)

     anand.prabhakar06@gmail.com

     Mobile No.:  +91-9989801551                                                                                                               

Career Objective

• A full-time position in biotechnology process development with a focus on mammalian cell culture

Professional Summary

·         More than 2 years of experience in mammalian cell culture early and late stage process development, scale-up/scale-down, and technology transfer to manufacturing plants, ATF system, ambr micro-bioreactor, guava flow-cytometer,  team based problem solving within the upstream cell culture

Educational Qualification

·         M.Tech. Chemical Engineering, IIT Bombay (2011)

·         B.Sc.(Engg) Chemical Engineering, BIT Sindri (2006)

Scholastic Achievement

·         All India ranking of 277/4194 candidates, Graduate Aptitude Test in Engineering-2009 (GATE-2009)

International Conference

·         "Effect of Endoplasmic Reticulum Stress On Protein Productivity In Recombinant CHO Cells" American Institute of Chemical Engineers (AIChe) Annual Meeting, Minneapolis Convention Center, MN, USA, October 2011

Current Job

(Feb 13-Present)

Scientist at Biologics Development Centre, Dr. Reddy's Laboratories

·         Mammalian cell upstream process development

·         Mammalian cell culture media formulation and feed strategies

·         In depth working knowledge of shake flask, micro-bioreactor (15 mL/20 mL ambr system), bench-top bioreactor function (3L/6.6L and 10L/13L) and ATF system

·         Production of proteins and monoclonal antibodies in shake flask, bench-scale/ambr bio-reactors/ATF2/ATF4 system as well as scale up

·         Process and technology transfer between sites

·         Scale-up and scale-down bioreactor studies and DOE

·         Applying statistical tools(JMP) for multivariate data analysis, DOE and process characterization

Previous Job

(Jan 12-Jan 13)

Worked asan Assistant Process Engineer in SPANOL CHEMICAL PVT. LTD. 

·         Working on the process development of Phosphate esters (using conventional and new process) and Solvent esters

·         Worked on catalyst improvement in the production of solvent esters

·         Working on the optimization of process condition and scale up of Tri n-butyl Phosphate production process from pilot plant to plant scale using Matlab

·         Conducted laboratory and pilot plant experiments to simulated actual plant conditions to improve yields of various existing products

(Nov 11-Jan 12)

Worked as Temporary Junior Research Fellow for the Sponsored Project “System Biology of Protein Secretion in Recombinant Mammalian Cell Lines” under Prof Sarika Mehra, IIT Bombay         

 (July 11-Sep 11)

DRF and Dean Technical Assistant (Additional) University of Petroleum Engineering and Studies, Dehradun

·         Course Instructor-Chemical Thermodynamics

Research Project & Seminar

       Modeling and Simulation of protein secretion pathway: M.Tech. Project       (Feb 2010- Jun 2011)

       Guide: Prof. Sarika Mehra, Chemical Engineering Department, IIT Bombay

• To understand the molecular mechanism behind protein folding and handling of stress inside ER

·         Dissected the biochemical reaction of protein secretion pathway into mass balance equations including unfolded protein response (UPR) within the endoplasmic reticulum(ER)

·         Developed a code in MATLAB and Mathematica at the molecular level based on the explicit Runge Kutta and GEAR package for solving ODE-IVPs

·         Identify the key factors controlling protein folding and secretion in mammalian cells

·         Analyze the key parameters which play a vital role to enhance the productivity of recombinant cell lines                      

Spontaneous Emulsification: M.Tech. Seminar                                                         (July 09-Nov 09)

       Guide: Prof. Rochish Thoakar, Chemical Engineering Department, IIT Bombay                           

·         Performed an extensive literature review of types and mechanism of spontaneous emulsification with O/W (oil and water) and W/O (water and oil) emulsification by PIT (phase inversion temperature) method

Course of Interest

Bioprocess Engineering principle Chemical Thermodynamics, Mass Tr. Operation, Heat Tr. Operation, Biochemical Engg, Chemical Reaction Engineering

Workshop

       “Introduction to Immunology for Physical Scientists and Engineers”

       (A Three days short-course, IIT Bombay)                                                            (Oct 25’ 10-Oct 27’ 10)

       Speaker: Prof. Arup K. Chakraborty, Chemical & Biological Engineering Dept., MIT. Ragaon     

       Institute of MGH

Course & B.Tech Projects

        Carbon and Nitrogen removal from Water                                                             (Jul 09 - Nov 09)

·         Aim of Project to survey kinetics & thermodynamics of chemical & biological removal of Nitrogen from water

  Flue gas conditioning for reducing suspended particulate matter from thermal power stations                                                                                                                                                                                                                                                                                (Jan 10 - Apr 10)

·         Studied to reduce suspended particulate matter from thermal power stations with the addition of Flue gas conditioning (FGC) also to increase efficiency of Electrostatic Precipitator (ESP)

       Model of the Unfolded Protein Response: Pancreatic β-Cell as a Case Study        (Jul 10 - Oct 12)

·         Studied the causes of aggregation of unfolded and misfolded protein and modify the kinetic model that minimizes the effect of misfolded and unfolded proteins

B.Tech. Project: Air Pollution & its Control. (Case Study: ACC Cement, SINDRI)                                                                                                                                                                                         (Jan 06 - May 06)

·         Studied four major Pollutants: NOx, SOx, SPM (Suspended Particulate Material) and CO

Summer Internship

       Five weeks practical training in Bokaro Steel Plant, SAIL (Jharkhand)                 (May 05 - June 05)

·         Coke Oven, By Product Plant, Sulfuric Acid Plant, Oxygen Plant, Acetylene Plant, Protective gas Plant-1, Protective gas Plant-2, Biological Oxidation Demand Plant (Water Treatment), R & D Lab, Blast Furnace, Safety & Environmental issues

Industrial Tour

·         INDAL Muri, Ranchi(Jharkhand)

·         BCCL Coal Washery, Sudamdih(Dhanbad)

·         ACC Cement, Sindri(Dhanbad)

Computer Skills

·         Software: JMP^®, ambr software, MATLAB, Mathematica, Matcont2.4, TechPlot360, Cell Designer 4.1, Latex, Ubuntu, EndnoteX4 (for bibliographies), Zotero, MS-office, COMSOL, Math Type6

Elective Courses

·         Bioprocess Principle, Computational methods in Chemical Engineering, Introduction to Biochemical Engineering, Introduction to System Biology, Polymer Reaction Engineering, Advanced Reaction Engineering, Advanced Thermodynamics, Advanced Transport Phenomena, Air Pollution and Control Technology, Experimental Lab Methods, Heat and Mass Transfer Operation, Petroleum Refining Engineering

Membership

·         Lifetime Alumini membership of IIT Bombay

·         Student membership of Indian Chemical Engineering Society                             (Apr 03 - May 06)

Interests

·         Yoga, Reading, Blogging and Travelling

Declaration

I hereby declare that the above written particulars are true to the best of my knowledge and belief

Place: Hyderabad                                                                                           Anand Prabhakar